analytical scientist discussion

Sitting with Scientists: LC-MS Guru-Seyed Sadjadi

Meet the Guru of Liquid Chromatography and Mass Spectrometry…Senior Scientist, Seyed Sadjadi. With 26 plus years in the clinical research industry, Mr. Sadjadi is known company-wide for his insights into Liquid Chromatography and Mass Spectrometry, or LC-MS. From working as a chemist in toxicology, to field applications for LC-MS small molecules, and even being here…

Method development and analyzing proteins

Transform Your HPLC and UHPLC Method Development Mindset on Analyzing Proteins— Including Monoclonal Antibodies!

A discussion of wide-pore/core-shell technology for monoclonal antibodies, method development considerations with wide-pore/core-shell columns, size exclusion chromatography (SEC) using sub-2 μm particles, and method optimization for SEC. (Webinar sneak peek) Click Here To View Entire Webinar   Introduction Analytical chemists have relied on high performance liquid chromatography (HPLC) columns such as Kinetex® Core-Shell and Luna®…

how much am I able to load on a reverse phase normal phase hplc column

Technical Tip: Column Loadability

Do you ever worry about your column’s loadability? Do you find yourself Googling, “How much am I able to load onto XYZ phase?” Ever fret over this issue so much you can’t get a wink of sleep at night? Well for preparative chromatographers, this might be the case. Column loadability directly influences the throughput and…

Effect of Flow Rate and Gradient Time in cannabis seperation phenomenex

Jeff Tries Cannabis Part 3: Method Development

Part 3: Effect of Flow Rate and Gradient Time Guest author: Jeff Layne Hey everybody, welcome back to my third blog article on cannabis HPLC method development. So far, we have done some preliminary gradient screening to narrow in an effective “gradient window” (75-100% acetonitrile in 10 min) and found that a range of different…

SPE vs LLE: A Battle of Methods

An Improved Clean Up and Recovery of Pharmaceutical Compounds from Plasma using Strata® -X In one corner, we have liquid-liquid extraction (LLE), which is perhaps the most established clean-up technique used in the chromatography field. Although it has been used for years, newer techniques with improved specificity towards particular analytes, have allowed analysts to improve…

Chiral chromatography pharmaceutical

What is Chiral?

What Exactly Does Chiral Mean? Chirality is a special physical property of a molecule which describes its geometry in three-dimensional space. Chiral molecules commonly consist of a carbon atom attached to four different substituents. The substituents can either be atoms or groups of atoms, but each must differ from the other three for the carbon…

mother's day in the lab - mom brain

“Mom Brain” is Real, and Science Can Back it

Attention fellow Momma-tographers! This is an open letter to those awesome moms out there suffering from…dun, dun, dun…MOM BRAIN! Do you find yourself leaving sample vials in the bathroom? Do you forget where you put your safety glasses when they are sitting on top of your head? Did you lose the word for the “sample-measuring-droppy-thingy”…