Effect of Flow Rate and Gradient Time in cannabis seperation phenomenex

Jeff Tries Cannabis Part 3: Method Development

Part 3: Effect of Flow Rate and Gradient Time Guest author: Jeff Layne Hey everybody, welcome back to my third blog article on cannabis HPLC method development. So far, we have done some preliminary gradient screening to narrow in an effective “gradient window” (75-100% acetonitrile in 10 min) and found that a range of different…

Jeff Tries Cannabis Part 2: HPLC Method Development

Blog 2: Effect of Different Mobile Phase Modifiers. Guest Author: Jeff Layne Welcome back to my series on method development for potency testing of naturally-occurring cannabinoids. In the previous article, I had done preliminary gradient screening of our 12 cannabinoids using a 100 x 4.6 mm Kinetex® C18 column and a series of water, acetonitrile,…

Testing for potency in Marijuana

Jeff Tries Cannabis: HPLC Method Development

Blog 1. Initial HPLC Gradient Screening for Potency Testing by LC-UV. Guest Author: Jeff Layne Hello everyone and welcome to the first of what I hope will be a series of articles on the topic of HPLC method development for cannabis-related analyses. My name is Jeff Layne, and I am head of the Product Management team…